Characterization of the nuclear polyhedrosis virus DNA of Adoxophyes orana and of Barathra brassicae
Identifieur interne : 004F92 ( Main/Exploration ); précédent : 004F91; suivant : 004F93Characterization of the nuclear polyhedrosis virus DNA of Adoxophyes orana and of Barathra brassicae
Auteurs : M. Jurkoví Ová [Pays-Bas] ; J. H. Van Touw [Pays-Bas] ; J. S. Sussenbach [Pays-Bas] ; J. Ter Schegget [Pays-Bas]Source :
- Virology [ 0042-6822 ] ; 1979.
English descriptors
- Teeft :
- Additional foldings, Adenovirus, Adenovirus type, Adoxophyes orana, Bacteriophage, Biol, Brassicae, Brussicae, Brussicue, Coli, Competition hybridization, Deoxyribonucleic acid, Different forms, Dna, Edta, Electron microscopy, Genome, Granulosis, Granulosis virus, Homology, Insect viruses, Kelly, Kinetics, Mandel, Marmur, Methods enzymol, Molecular weight, Molecular weights, Nuclear polyhedrosis virus, Nuclear polyhedrosis viruses, Nucleic acids, Orana, Oruna, Orunu, Pathol, Polyhedral, Polyhedron, Polyhedrosis, Reaction mixture, Reassociation, Reassociation kinetics, Reassociation mixture, Repetitive sequences, Sequence homology, Sodium phosphate buffer, Thermal denaturation, Unlabelled, Unlabelled fragments, Virology, Virus, Virus particles.
Abstract
Abstract: Circular double-stranded DNA was isolated from nuclear polyhedrosis virus (NPV) of Adoxophyes orana (virus particles singly embedded in the polyhedral matrix) and NPV of Barathra brassicae (virus particles multiply embedded in the polyhedral matrix), and some of their physical properties were determined. The molecular weights of A. orana and B. brassicae NPV-DNA, 6.7 x 107 and 8.9 x 107, respectively, were determined by electron microscopy and by renaturation kinetics analysis. The latter analysis also showed that both genomes do not contain repetitive sequences. Absence of homology between DNA of these two viruses was shown by competition hybridization of A. orana NPV-DNA with B. brassicae NPV-DNA. Analysis of these DNAs with the restriction endonuclease EcoRI confirmed that they are different. The buoyant densities in CsCl of A. orana NPV-DNA and of B. brassicae NPV-DNA, 1.694 and 1.696 g/cm3, respectively, are consistent with (G + C) contents of 34.5 and 37%, respectively, as determined by thermal denaturation.
Url:
DOI: 10.1016/0042-6822(79)90271-X
Affiliations:
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Ter Schegget</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:105E991840D539CF7B35062DC732A2BF88886ACC</idno><date when="1979" year="1979">1979</date><idno type="doi">10.1016/0042-6822(79)90271-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-45TGLNPR-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001D88</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D88</idno><idno type="wicri:Area/Istex/Curation">001D88</idno><idno type="wicri:Area/Istex/Checkpoint">002441</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002441</idno><idno type="wicri:doubleKey">0042-6822:1979:Jurkovi Ova M:characterization:of:the</idno><idno type="wicri:Area/Main/Merge">005072</idno><idno type="wicri:Area/Main/Curation">004F92</idno><idno type="wicri:Area/Main/Exploration">004F92</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Characterization of the nuclear polyhedrosis virus DNA of Adoxophyes orana and of Barathra brassicae</title><author><name sortKey="Jurkovi Ova, M" sort="Jurkovi Ova, M" uniqKey="Jurkovi Ova M" first="M." last="Jurkoví Ová">M. Jurkoví Ová</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Virology, State Agricultural University, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Van Touw, J H" sort="Van Touw, J H" uniqKey="Van Touw J" first="J. H." last="Van Touw">J. H. Van Touw</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Virology, State Agricultural University, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Sussenbach, J S" sort="Sussenbach, J S" uniqKey="Sussenbach J" first="J. S." last="Sussenbach">J. S. Sussenbach</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Ter Schegget, J" sort="Ter Schegget, J" uniqKey="Ter Schegget J" first="J." last="Ter Schegget">J. Ter Schegget</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory for Hygiene, Amsterdam</wicri:regionArea><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1979">1979</date><biblScope unit="volume">93</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="8">8</biblScope><biblScope unit="page" to="19">19</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Additional foldings</term><term>Adenovirus</term><term>Adenovirus type</term><term>Adoxophyes orana</term><term>Bacteriophage</term><term>Biol</term><term>Brassicae</term><term>Brussicae</term><term>Brussicue</term><term>Coli</term><term>Competition hybridization</term><term>Deoxyribonucleic acid</term><term>Different forms</term><term>Dna</term><term>Edta</term><term>Electron microscopy</term><term>Genome</term><term>Granulosis</term><term>Granulosis virus</term><term>Homology</term><term>Insect viruses</term><term>Kelly</term><term>Kinetics</term><term>Mandel</term><term>Marmur</term><term>Methods enzymol</term><term>Molecular weight</term><term>Molecular weights</term><term>Nuclear polyhedrosis virus</term><term>Nuclear polyhedrosis viruses</term><term>Nucleic acids</term><term>Orana</term><term>Oruna</term><term>Orunu</term><term>Pathol</term><term>Polyhedral</term><term>Polyhedron</term><term>Polyhedrosis</term><term>Reaction mixture</term><term>Reassociation</term><term>Reassociation kinetics</term><term>Reassociation mixture</term><term>Repetitive sequences</term><term>Sequence homology</term><term>Sodium phosphate buffer</term><term>Thermal denaturation</term><term>Unlabelled</term><term>Unlabelled fragments</term><term>Virology</term><term>Virus</term><term>Virus particles</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Circular double-stranded DNA was isolated from nuclear polyhedrosis virus (NPV) of Adoxophyes orana (virus particles singly embedded in the polyhedral matrix) and NPV of Barathra brassicae (virus particles multiply embedded in the polyhedral matrix), and some of their physical properties were determined. The molecular weights of A. orana and B. brassicae NPV-DNA, 6.7 x 107 and 8.9 x 107, respectively, were determined by electron microscopy and by renaturation kinetics analysis. The latter analysis also showed that both genomes do not contain repetitive sequences. Absence of homology between DNA of these two viruses was shown by competition hybridization of A. orana NPV-DNA with B. brassicae NPV-DNA. Analysis of these DNAs with the restriction endonuclease EcoRI confirmed that they are different. The buoyant densities in CsCl of A. orana NPV-DNA and of B. brassicae NPV-DNA, 1.694 and 1.696 g/cm3, respectively, are consistent with (G + C) contents of 34.5 and 37%, respectively, as determined by thermal denaturation.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Hollande-Septentrionale</li><li>Utrecht (province)</li></region><settlement><li>Amsterdam</li><li>Utrecht</li></settlement></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Jurkovi Ova, M" sort="Jurkovi Ova, M" uniqKey="Jurkovi Ova M" first="M." last="Jurkoví Ová">M. Jurkoví Ová</name></noRegion><name sortKey="Sussenbach, J S" sort="Sussenbach, J S" uniqKey="Sussenbach J" first="J. S." last="Sussenbach">J. S. Sussenbach</name><name sortKey="Ter Schegget, J" sort="Ter Schegget, J" uniqKey="Ter Schegget J" first="J." last="Ter Schegget">J. Ter Schegget</name><name sortKey="Van Touw, J H" sort="Van Touw, J H" uniqKey="Van Touw J" first="J. H." last="Van Touw">J. H. 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